ジャーナル
/
神経科学
/
Ultrasound-Guided Microiniezione nel proencefalo mouse In Utero A E9.5
JoVE Journal
神経科学
This content is Free Access.
JoVE Journal 神経科学
Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5
Please note that all translations are automatically generated. Click here for the English version.

Ultrasound-Guided Microiniezione nel proencefalo mouse In Utero A E9.5

17,589 Views

•

07:47 min

•

November 13, 2010

DOI:

10.3791/2047-v

DOI:
10.3791/2047-v

•

07:47 min
November 13, 2010

•

7 Views
,
1Institute for Cell Engineering Neuroregeneration and Stem Cell Programs,Johns Hopkins University School of Medicine, 2Departments of Neurology, Neuroscience, and Oncology,Johns Hopkins University School of Medicine

概要

Automatically generated

Nella chirurgia in utero sopravvivenza in topi permette la manipolazione molecolare dell'espressione genica durante lo sviluppo. Qui si descrive l'utilizzo di alta frequenza ecografia per guidare l'iniezione di vettori retrovirali nel cervello di topo al giorno embrionale (E) 9.5.

Explore More Videos

Ultrasound-guidedMicroinjectionMouse ForebrainIn UteroE9.5Survival SurgeryGene Expression胚発生Microinjection NeedleRetroviral VectorsVentricular SystemLaparotomySaline BathInjected EmbryosAmniotic FluidNeural TubeBrain RegionsViral InfectionCell Transplantation

Read Article

Cite this Article

Pierfelice, T. J., Gaiano, N. Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5. J. Vis. Exp. (45), e2047, doi:10.3791/2047 (2010).

Download .ris file

Copy

Share Video

.

Copy