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Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5

Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5

17,617 Views

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07:47 min

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November 13, 2010

DOI:

10.3791/2047-v

DOI:
10.3791/2047-v

•

07:47 min
November 13, 2010

•

7 Views
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1Institute for Cell Engineering Neuroregeneration and Stem Cell Programs,Johns Hopkins University School of Medicine, 2Departments of Neurology, Neuroscience, and Oncology,Johns Hopkins University School of Medicine

Summary

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In utero survival surgery in mice permits the molecular manipulation of gene expression during development. Here we describe the use of high-frequency ultrasound imaging to guide the injection of retroviral vectors into the mouse brain at embryonic day (E) 9.5.

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Ultrasound-guidedMicroinjectionMouse ForebrainIn UteroE9.5Survival SurgeryGene ExpressionEmbryogenesisMicroinjection NeedleRetroviral VectorsVentricular SystemLaparotomySaline BathInjected EmbryosAmniotic FluidNeural TubeBrain RegionsViral InfectionCell Transplantation

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Pierfelice, T. J., Gaiano, N. Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5. J. Vis. Exp. (45), e2047, doi:10.3791/2047 (2010).

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