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Ultrasound-Guided micro-injectie in de muis voorhersenen In Utero At E9.5
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Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5
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Ultrasound-Guided micro-injectie in de muis voorhersenen In Utero At E9.5

17,589 Views

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07:47 min

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November 13, 2010

DOI:

10.3791/2047-v

DOI:
10.3791/2047-v

•

07:47 min
November 13, 2010

•

7 Views
,
1Institute for Cell Engineering Neuroregeneration and Stem Cell Programs,Johns Hopkins University School of Medicine, 2Departments of Neurology, Neuroscience, and Oncology,Johns Hopkins University School of Medicine

概要

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In utero overleven chirurgie bij muizen laat de moleculaire manipulatie van genexpressie tijdens de ontwikkeling. Hier beschrijven we het gebruik van hoge-frequentie ultrasonografie om de injectie van retrovirale vectoren gids in de muis hersenen op embryonale dag (E) 9.5.

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Cite this Article

Pierfelice, T. J., Gaiano, N. Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5. J. Vis. Exp. (45), e2047, doi:10.3791/2047 (2010).

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