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OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
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生物学
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JoVE Journal 生物学
OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides

OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides

DOI:
10.3791/2046-v

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08:43 min

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June 20, 2010

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, ,
1Energy Biosciences Institute,University of California, Berkeley, 2Department of Plant and Microbial Biology,University of California, Berkeley

章

  • 00:00標題
  • 00:45Introduction
  • 01:23Solubilisation of Polymers from Isolated Cell Walls
  • 03:50MALDI-TOF MS Analysis of the Released Oligosaccharides
  • 05:42In situ OLIMP Analysis
  • 07:19OLIMP Representative Results and Analysis
  • 08:18Conclusion

概要

自動翻訳

自動翻訳

A rapid way is described to gain insights into the structure of polysaccharides in an extracellular matrix. The method takes advantage of the specificity of glycosylhydrolases and the sensitivity of mass spectrometry allowing minute amounts of materials to be analyzed. This technique is adaptable to be used directly on tissue itself.

タグ

OLIgo Mass ProfilingExtracellular PolysaccharidesGlycoproteinsProteoglycansPolysaccharidesExtracellular MatrixGlycostructuresCell-to-cell CommunicationCell-to-environment CommunicationSugar MoietiesPlant Cell WallsBiopolymersCarbon-neutral Renewable ResourcePetrochemicalsFossil FuelFuel ConversionEnzymatic DegradationChemical DegradationGlycostructures AnalysisOLIgo Mass Profiling Method

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