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Method for the Isolation of Francisella tularensis Outer Membranes
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June 29, 2010
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IsolationFrancisella TularensisOuter MembranesGram-negativeCoccobacillusZoonotic DiseaseTularemiaInfectious DoseVirulence FactorsOuter Membrane Proteins (OMPs)Host Cell BindingEntryIntracellular SurvivalVirulenceImmune EvasionProtective VaccinesGram-negative BacteriaBulk Membrane Extraction TechniquesSonicationCentrifugationDetergent ExtractionPeriplasmic ContaminantsCytoplasmic ContaminantsInner Membrane (IM)SpheroplastingOsmotic LysisSucrose Density Gradient CentrifugationLipopolys
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Huntley, J. F., Robertson, G. T., Norgard, M. V. Method for the Isolation of Francisella tularensis Outer Membranes. J. Vis. Exp. (40), e2044, doi:10.3791/2044 (2010).
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