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Optimizing Low-Frequency Mutation Detection with Wild-Type Blocking PCR
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Blocker Displacement Amplification-Enhanced Sanger Sequencing for Low-Frequency Mutation Detection in Cancer Tissues
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JoVE Journal 遺伝学
Blocker Displacement Amplification-Enhanced Sanger Sequencing for Low-Frequency Mutation Detection in Cancer Tissues

Blocker Displacement Amplification-Enhanced Sanger Sequencing for Low-Frequency Mutation Detection in Cancer Tissues

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03:06 min

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August 23, 2024

DOI:

10.3791/202183-v

DOI:
10.3791/202183-v

•

03:06 min
August 23, 2024

•

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1Kindstar Global Technology Inc., 2Yongzhou Central Hospital

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遺伝学Sanger sequencingWTB-PCRhigh sensitivitylow-frequency mutationsAITLRHOA G17V

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Blocker Displacement Amplification-Enhanced Sanger Sequencing for Low-Frequency Mutation Detection in Cancer Tissues. J. Vis. Exp. (Pending Publication), e202183, doi:10.3791/202183 (2024).

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