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Evaluating Calcium Activity in Retinal Ganglion Cells Layer
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Rat Retina Excision and Mounting on Porous Membrane for Calcium Imaging
JoVE Journal
神経科学
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JoVE Journal 神経科学
Rat Retina Excision and Mounting on Porous Membrane for Calcium Imaging

Rat Retina Excision and Mounting on Porous Membrane for Calcium Imaging

Evaluating Calcium Activity in Retinal Ganglion Cells Layer

DOI:
10.3791/200529-v

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02:42 min

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August 18, 2023

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, , , , , , , , ,
1Institut de Ciències Fotòniques (ICFO),The Barcelona Institute of Science and Technology, 2Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST,Campus UAB, 3Institut de Física d’Altes Energies (IFAE),The Barcelona Institute of Science and Technology, Campus UAB, 4Institució Catalana de Recerca i Estudis Avançats (ICREA)

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Rat RetinaCalcium ImagingVitreous InjectionAAV2-CAG-GCaMP5GFundoscopyOCTGanglion Cell LayerRetina ExcisionFilter PaperAIMS MediumEyeballAnterior SegmentStraight ForcepsIncisionLensScleraOptic NervePorous MembraneMicro Electrode ArrayMEA

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