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Assessing Hepatic Steatosis with 3D Fluorescence Lipid Droplet Reconstruction

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Frozen Liver Tissue Sectioning and BODIPY Staining of Lipid Droplets

JoVE Journal
生化学

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JoVE Journal 生化学

Frozen Liver Tissue Sectioning and BODIPY Staining of Lipid Droplets

Frozen Liver Tissue Sectioning and BODIPY Staining of Lipid Droplets

Assessing Hepatic Steatosis with 3D Fluorescence Lipid Droplet Reconstruction

DOI:

10.3791/200052-v

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01:18 min

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June 02, 2023

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Karina Garcia^2,3,4, André Alves^2,3, Teresa M. Ribeiro-Rodrigues^2,3, Flávio Reis^2,3, Sofia Viana^2,3,4

¹Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine,University of Coimbra, ²Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, ³Clinical Academic Center of Coimbra (CACC), ⁴Polytechnic Institute of Coimbra,Coimbra Health School

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Cryostat Sectioning BODIPY Staining Lipid Droplets PBS Wash BODIPY 493/503 DAPI Fluorescence Microscopy Coverslip Mounting

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