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技术成像钙 2 +信号在人类精子
JoVE Journal
生物学
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JoVE Journal 生物学
Techniques for Imaging Ca2+ Signaling in Human Sperm
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技术成像钙 2 +信号在人类精子

16,930 Views

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07:38 min

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June 16, 2010

DOI:

10.3791/1996-v

DOI:
10.3791/1996-v

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07:38 min
June 16, 2010

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12 Views
, , , , , , , ,
1School of Biosciences,University of Birmingham, 2School of Medicine,University of Birmingham, 3Centre for Human Reproductive Science,Birmingham Women’s Hospital

概要

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刺激诱发的[Ca<sup> 2 +</sup>]：我个人的人类精子的信号进行评估。运动型细胞加载与Ca<sup> 2 +</sup>敏感的荧光染料（AM -酯法），并固定在perfusable室。时间推移荧光显微镜和细胞成像，通过灌注介质刺激。单细胞的反应（或地区）使用Excel进行离线分析。

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Cite this Article

Nash, K., Lefievre, L., Peralta-Arias, R., Morris, J., Morales-Garcia, A., Connolly, T., Costello, S., Kirkman-Brown, J. C., Publicover, S. J. Techniques for Imaging Ca2+ Signaling in Human Sperm. J. Vis. Exp. (40), e1996, doi:10.3791/1996 (2010).

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