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Techniques for Imaging Ca2+ Signaling in Human Sperm
JoVE 杂志
生物学
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JoVE 杂志 生物学
Techniques for Imaging Ca2+ Signaling in Human Sperm

Techniques for Imaging Ca2+ Signaling in Human Sperm

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07:38 min

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June 16, 2010

DOI:

10.3791/1996-v

DOI:
10.3791/1996-v

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07:38 min
June 16, 2010

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1School of Biosciences,University of Birmingham, 2School of Medicine,University of Birmingham, 3Centre for Human Reproductive Science,Birmingham Women’s Hospital

Tags

Fluorescence MicroscopyCa2+ SignalingHuman SpermFluorescent DyesLoading MethodPhysiological StimulationCapacitating ConditionsAM EsterCytoplasmVisible Wavelength DyesRatiometric RecordingAdvantages And DisadvantagesImaging ChamberPoly-D-lysine Coated CoverslipPerfusion ApparatusTime-lapse AcquisitionRegions Of InterestData Normalization

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Nash, K., Lefievre, L., Peralta-Arias, R., Morris, J., Morales-Garcia, A., Connolly, T., Costello, S., Kirkman-Brown, J. C., Publicover, S. J. Techniques for Imaging Ca2+ Signaling in Human Sperm. J. Vis. Exp. (40), e1996, doi:10.3791/1996 (2010).

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