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Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging
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March 15, 2010
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This protocol describes three Drosophila preparations: 1) adult brain dissection, 2) adult retina dissection and 3) developing eye disc- brain complexes dissection. Emphasis is laid on special preparation techniques and conditions for live imaging, although all preparations can be used for fixed tissue immunohistochemistry.
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Williamson, W. R., Hiesinger, P. R. Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging. J. Vis. Exp. (37), e1936, doi:10.3791/1936 (2010).
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