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Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
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April 14, 2010
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概要
Automatically generated
This procedure describes a quick and easy workflow to introduce siRNA into difficult to transfect cell lines and follow gene expression by real-time PCR. Use of an automated cell counter, multi-well electroporation plate, and automated electrophoresis station provide quick and reliable results without the need for expensive robotic handling.
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Automated Cell CounterGene Expression StudiesSiRNA KnockdownIL-4 Dependent Gene ExpressionNamalwa CellsCellular ModelsElectroporationNucleic Acids TransfectionSignaling PathwayExperimental StagesPost Transfection Real-time PCR Gene Expression AnalysisTranscriptional Activator STAT6CCL17Burkitt Lymphoma Cell Line
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McCoy, A. M., Litterst, C., Collins, M. L., Ugozzoli, L. A. Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells. J. Vis. Exp. (38), e1904, doi:10.3791/1904 (2010).
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