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12:10 min
December 08, 2009
DOI:
10.3791/1553-v
我们展示了为生成诱导多能干的干细胞,人类体细胞用慢病毒介导的人为因素OCT4,SOX2,NANOG和Lin28交付协议。多能性证实了形态学和胚胎干(ES)细胞特异性标志物的存在。
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Wu, D., Hamilton, B., Martin, C., Gao, Y., Ye, M., Yao, S. Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set. J. Vis. Exp. (34), e1553, doi:10.3791/1553 (2009).
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