The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
Balbo, A., Zhao, H., Brown, P. H., Schuck, P. Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell. J. Vis. Exp. (33), e1530, doi:10.3791/1530 (2009).