This protocol demonstrates how to provide practical meaningful environmental enrichment for pigs which are housed on slatted flooring during the different stages of their lives, and how to collect saliva samples in a non-invasive manner for the measurement of cortisol concentrations, as a biomarker for acute stress.
As pork is the most consumed meat worldwide, the welfare of animals in the swine industry has increasingly become a major public concern, which imposes a substantial pressure coming from customers, legislators and other stakeholders, to make management changes to improve the well-being of these animals. Several studies have demonstrated that providing environmental enrichment to pigs allows them to express their natural behavior, such as rooting and exploring, as well as nesting prior to farrowing, and is associated with reduced stress and improved production and welfare. However, many considerations should be taken into account when providing environmental enrichment, such as the type of floor, drainage and sewage systems, the pigs' stage in life, the material, as well as its hanging method, height and location within the pen. The objectives of this paper are (1) to give methodologic information on how to provide a relatively simple and practical meaningful environmental enrichment for pigs which are housed on slatted floors during the different stages of their life, and (2) to demonstrate how to collect saliva samples for the measurement of cortisol concentrations, as a biomarker for acute stress. Protocols include information regarding the use of jute, cotton ropes, straw in racks, as well as chewable silicone sticks devices as environmental enrichment in pens of farrowing and lactation, weaners and finishers. In addition, the use of cotton rope for a non-invasive saliva samples collection for cortisol concentrations analysis is detailed. The protocols provided are relevant for professionals aiming to improve and monitor animal welfare, in both research and industrial swine farming.
Pork is the most consumed meat worldwide, with over 1.3 billion pigs being raised and slaughtered annually1,2. In recent years, the welfare of animals in the swine industry has increasingly become a major public concern, which imposes substantial pressure, coming from customers, legislators and other stakeholders, to make management changes to improve the well-being of these animals. Several studies demonstrated that providing environmental enrichment to pigs is associated with reduced stress and improved production and welfare, as it allows the pigs to express their natural behavior, such as rooting and exploring, as well as nesting prior to farrowing3,4,5,6,7.
Pigs are considered to be intelligent animals, and have a highly inquisitive nature; thus, if a suitable environment is not provided, pigs will likely demonstrate stereotypic behavior and direct manipulative social behavior towards pen mates, which may lead to tail biting, as well as other injuries and stress8,9. Therefore, providing a meaningful environmental enrichment is advised by professionals, and in some countries even imposed by regulations and legislations, such as the European Union Council Directive 2008/120/EC5.
Providing meaningful environmental enrichment can be challenging; it should fulfill the natural behavioral needs of the pigs in each stage of their lives, as well as take into consideration practical and technical limitations. Prior to farrowing, providing nesting material is associated with shorter farrowing duration as well as higher survival rate of the newborn piglets during farrowing and throughout the lactation period. Moreover, in free pens with environmental enrichment, maternal behavior is improved, as well as the cognitive performance and weight gain of the piglets3,10,11,12,13. After weaning, grouping pigs (weaners, growing or finishers) from different litters or pens can be stressful and cause aggressiveness towards other pen mates, which may lead to injuries14,15. Therefore, when mixing unfamiliar pigs, providing meaningful environmental enrichment can potentially reduce the occurrence of undesired behavior resulting from frustration and aggressiveness or redirection of rooting behavior.
According to the European Union Council Directive 2008/120/EC (established in 2001/93/EC), it is required that pigs have permanent access to a sufficient quantity of material, to enable proper investigation and manipulation activities, such as straw, hay, wood, sawdust, mushroom compost, peat or a mixture of these5,6. However, the use of these materials may be unmanageable on many farms, as it may cause blockage of the drainage and sewage systems, particularly on farms with slatted flooring. Still, according to the EU Council Directive, farmers can provide alternative enrichment materials, as long as it allows the pigs to perform proper investigation and manipulation activities.
Lack of environmental enrichment may potentially lead to frustration and stress3, which may activate the hypothalamic pituitary adrenal (HPA) axis. In pigs, as well as in humans and other animals, stress typically leads to the secretion of adrenocorticotropic hormone (ACTH) from the pituitary gland. ACTH binds to its receptors on the adrenal cortex and stimulates adrenal release of the glucocorticoid cortisol, which is considered as a major biomarker of stress and can be measured in blood, urine, saliva and hair. Saliva cortisol is a marker for acute stress, since it reflects its biologically active concentration in the blood4. It has the advantage of a non-invasive sample collection, without handling of the animals, by means of cotton ropes provided as environmental enrichment16,17. Hair cortisol is being used as a marker for chronic stress, since cortisol accumulates in the hair over time and can be extracted and measured18,19; however, it requires animal handling, the number of repeated sampling is limited by the hair growth rate, and the analysis is more cumbersome as it require long extraction process. Still, both saliva and hair cortisol may provide complementary information to asses animal welfare.
The objectives of this paper are (1) to give methodologic information on how to provide relatively simple and practical meaningful environmental enrichment for pigs which are housed on slatted floor during different stages of their lives (farrowing and lactation, weaners to finishers), and (2) to demonstrate how to non-invasively collect saliva samples for the measurement of cortisol concentrations, as a biomarker for acute stress.
The study protocol was ethically approved by the Hebrew University's Institutional Animal Care and Use Committee (MD-16-14754-2). The study was conducted during 2017, at Lahav Animal Research Institute and the Hebrew University of Jerusalem, Israel.
1. Environmental enrichment protocol during farrowing and lactation period
NOTE: In this study, sows were housed in farrowing/lactation pens, under restraint for a limited time, from 3-5 days prior to the expected farrowing date and up to 10 days post-farrowing; thereafter, confinement bars were removed, to allow free movement and interaction between the sow and its piglets (illustrated in Figure 1A). However, the following protocol is also suitable for conventional farrowing crates, in which the sow is restrained throughout the entire lactation period.
2. Environmental enrichment protocol for weaners (when straw cannot be provided)
NOTE: In this study, after weaning, each two litters were grouped into one pen of weaners. Optimally, groups should remain static until slaughter, without mixing or introducing new pen mates into the pen, to avoid hierarchy-related struggles and injuries. Space allowance in the pen was according to European Council Directive 2008/120/EC and the Israeli legislation, with approximately 20 pigs per group, and 0.3 m2 of unobstructed floor area available per pig. For technical reasons, straw could not be provided for weaners, not even in racks. When straw can be provided, use the finishers' protocol from weaning to slaughter, as detailed in protocol section 3.
3. Environmental enrichment protocol for finishers
NOTE: When straw can be provided during the whole period, start using this protocol from weaning until slaughter, instead of in the finishers' pens only.
4. Saliva samples collection for cortisol concentrations analysis
NOTE: This section provides information how to non-invasively collect saliva for cortisol concentration analysis, as a biomarker for acute stress; however, saliva samples can also be used for analysis of other biomarkers and even for screening of potential pathogens. In addition, hair cortisol should be measured when applicable.
In the current study, 16 litters (170 piglets) were allocated randomly into two treatment groups; in one group, environmental enrichment was provided to eight litters ("Enriched Group", as described in protocol sections 1-3), while it was not provided to the other eight litters ("Non-enriched Group"). After weaning, each two litters were grouped into one group of about 20 pigs. Saliva samples were collected and analyzed for cortisol concentrations every two weeks in each pen, as described in protocol section 4.
In this study, when provided properly, sows, piglets and pigs showed interest in all enrichment devices. Sows used the jute and cotton ropes prior to farrowing, when provided. During the lactation period, when jute and cotton ropes were reachable for both the dam and her piglets, piglets imitated their dams from the age of 2-3 days old; however, when materials were not reachable for the sow, piglets started using the material later, typically after more than one week of age. In lactation and weaners' pens, the jute and cotton ropes were usually replaced or refilled every 4-5 days, while the chewable silicone sticks were replaced only once, when pigs were moved from weaners' pen to finishers' pen, at the age of 70 days. In the finishers' pens, no drainage and sewage blockages were recorded.
The odds to die during the whole period until slaughter was significantly higher among piglets/pigs in the non-enriched group as compared to the enriched group (OR = 3.38, 95% CI = 1.05-10.83; P = 0.004). Saliva cortisol samples were successfully collected during the study every two weeks, just before the second feeding of the day. The pigs in the enriched group showed high interest in the saliva collecting cotton ropes from the beginning; however, in the non-enriched group saliva volumes in the initial collection were typically lower (2-10 mL), but from the second or third collection sessions it was similar between the groups. Typically, a volume larger than 30-40 mL was easily extracted from each rope. Samples were stored frozen (-80 °C) until analysis. Cortisol concentrations, as measured by ELISA, were overall significantly lower in the enriched pigs, as compared to the non-enriched pigs (P = 0.0044; Figure 4).
Figure 1: Environmental enrichment during farrowing and lactation period. (A) Designed farrowing pen illustration which demonstrates two stages of the confinement bars, either when the sow is restrained (stage R) or after confinement bars are removed (stage F) to allow free movement and interaction between the sow and her piglets. Suggested locations for the jute and cotton ropes before and after farrowing are marked in green and blue, respectively. (B) Images of a sow and her piglets interact with cotton ropes (CR) and jute (J) provided as environmental enrichment in a farrowing/lactation pen. Please click here to view a larger version of this figure.
Figure 2: Environmental enrichment for weaners and finishers. (A) A schematic illustration of a pen demonstrating the suggested locations for hanging the environmental materials in the "active area" (blue X). (B–C) Images of pigs enriched as suggested. (B) In the weaners' pen, cotton ropes (CR) provided as environmental enrichment or for saliva samples collection hung from the ceiling or from a pole (P) to distance it from the pen's wall, chewable silicone sticks device (BR) hung by a chain from the ceiling (HC). (C) In the finishers' pen, straw is provided in a rack (SR), a straw tower (ST) or a straw basket (SB); a mat (M) can cover the slatted floor underneath each of these straw devices. Please click here to view a larger version of this figure.
Figure 3: Saliva sampling process using cotton ropes. (A–F) The six main stages for the saliva sampling are illustrated: hanging the rope, unraveling the free end of the rope, allowing the pigs to chew it, saliva extraction from the rope into a plastic bag and then transferring it to a plastic tube. Please click here to view a larger version of this figure.
Figure 4: Saliva cortisol concentrations in enriched and non-enriched pigs. Saliva samples were collected every two weeks using cotton ropes, at the pens' level, from weaning to slaughter, from enriched and non-enriched pigs; cortisol concentrations were measured by ELISA. For this analysis, all samples were clustered together per group, without taking the sampling date into consideration. Cortisol concentrations were significantly lower in the Enriched Group, as compared to the Non-enriched Group (Mann Whitney test; P = 0.0044). Results are presented as mean ± SEM. Please click here to view a larger version of this figure.
Herein, we describe protocols detailing how to provide simple and practical environmental enrichment for pigs which are housed on slatted floor during different stages of their life, and how to non-invasively collect saliva samples for the measurement of cortisol concentrations, as a biomarker for acute stress. Straw as a bedding is considered as one of the most suitable environmental enrichment for pigs, but may be impractical on slatted floors. However, relatively inexpensive alternatives such as jute, cotton ropes, straw in racks, and chewable devices can practically and safely be applied.
The range of saliva cortisol concentrations in this study are comparable to previous publications17,22,23. The significant differences in saliva cortisol concentrations between enriched- and non-enriched animals, is in accordance with the hypothesis that environmental enrichment may reduce acute stress in pigs at a point sample, as has been shown in previous studies, at least up to a certain age, as long as the circadian rhythm is not damaged by the prolonged chronic stress24,25. As noted, cortisol can be measured in blood, urine, and saliva as markers for acute stress, and in hair as a marker for chronic stress. A protocol for measuring hair cortisol was previously described by Meyers et al. and may be relevant when assessing animal welfare19. However, when looking for markers for acute stress, one should consider that any sampling, by itself, may increase cortisol release due to animal handling or pain caused during the procedure16,26. Hence, non-invasive saliva sampling, as suggested in the current study, is a relevant method, which can be used for cortisol analysis, as well as for the analysis of other biomarkers and even for screening of potential pathogens27.
The main challenges in collecting saliva according to this protocol may be when collecting it from pigs that are unfamiliar with the ropes, as they may avoid chewing the ropes, and therefore, training may be needed. Training can be performed by using ropes which are soaked in apple juice (in such cases, those saliva samples cannot be used for analyses). If saliva samples are needed from individually housed pigs, the collection may be even more challenging than from a group, and training sessions with apple juice-soaked ropes are typically required. Chewing the ropes may also be encouraged by sampling individuals next to each other due to mimicking. For the training of young piglets, ropes should be hung to be reachable for their mother to encourage learning and early chewing. Another possible obstacle when using cotton ropes for enrichment or for saliva collection is that it can be caught in ear tags of certain brands. If it occurs, either the ear tags or the enrichment method has to be replaced.
Using cotton ropes allows measuring saliva cortisol concentration at the group level, which provides applicable screening for this hormone. A possible disadvantage of this collection method is that it may not be a representative measurement of all individuals in the group, as it is affected by the amount of saliva absorbed in the rope from each individual pig. In order to minimize this disadvantage, it is advised to provide adequate number of ropes, to allow sufficient collection time, as well as to perform several repeated collections. As compared to the existing method of individually sampling, the collection itself is not stressful for the pigs, since it is given as an environmental enrichment, without handling of the pigs.
In summary, pigs can be environmentally enriched at all life stages, even on slatted floor, using jute, cotton ropes, straw in racks and chewable devices. The type of enrichment, the hanging method, as well as the height and location within the pen, are important for their effectiveness. Regarding saliva collection by cotton topes, the timing and the hanging recommendations are crucial for successful samplings. These protocols are relevant for professionals aiming to improve and monitor animal welfare, in both research and industrial swine farming.
The authors have nothing to disclose.
We thank Lahav CRO for conducting the research in their farm and to the farm's team for providing valuable technical help throughout the study. The study was funded by the Israel Chief Scientist, Ministry of Agriculture and Rural Development.
Bite-Rite | Ikadan System USA Inc. | Consider ordering additional replaceable silicone sticks | |
ELISA; Saliva Cortisol Kit | DRG International Inc. NJ, USA | Slv2930 | |
HALM 60/80 CM | W. Domino A/S, DK | 49084/ 85 | |
TEGO Swine Oral Fluids Kit | ITL BioMedical, USA | A100930 | Including everything needed for the saliva sampling protcol |