This video demonstrates a method for tracking bispecific antibody-induced T cell trafficking to tumors in a mouse model. Luciferase-transduced effector T cells are injected into a mouse with a subcutaneous tumor cell xenograft. Bispecific antibodies are then introduced, binding to T cells and the tumor cells. The localization of the T cells is tracked using bioluminescent imaging, with the magnitude of the signal indicating the extent of T cell infiltration into the tumor.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Transfection of 293T cells with luciferase and harvest of viral supernatant Culture of 293T cells Prepare media by adding the following to a liter of Dulbecco's modified Eagle medium (DMEM) each: 110 mL of heat-inactivated fetal bovine serum (FBS), 11 mL of penicillin-streptomycin. Thaw 5 x 10…