Calvarial Window Generation in Mouse Model: A Surgical Procedure to Create Window in the Top Part of the Mouse Skull

Published: April 30, 2023

Abstract

Source: Geisler, J. A. et al. Modeling Brain Metastases Through Intracranial Injection and Magnetic Resonance Imaging. J. Vis. Exp. (2020)

In this video, we describe the surgical procedure to create a calvarial window for accessing the brain of a mouse model.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Prepare Stereotactic Units for Surgery Turn on all anesthesia machines, digital Vernier scales, and digital injectors. NOTE: All surgical tools should be adequately cleaned and sterilized prior to surgery. Utilize anesthetic machines with an induction chamber attachment in a biological …

Representative Results

Figure 1: Stereotactic and anesthesia systems. (A) Anesthesia induction chamber setup within a biological safety cabinet. (B) Stereotactic anesthetic delivery setup highlighting anesthesia tubing from the anesthetic machine to the nose cone on the stereotactic apparatus (see inset in (C)). Green arrows indicate delivered anesthetic gas tubing, and blue arrows indicate scavenged gas tubing. (…

開示

The authors have nothing to disclose.

Materials

Surgical Materials
Betadine Purdue Products 19-027132 Povidone-iodine, 7.5%
Bone Wax Surgical Specialities 903 Sterile and malleable beeswax and isopropyl palmitate
Buponorphine SR-Lab ZooPharm N/A Long acting injectable analgesic 5 mL (0.5 mg/mL) polymetric formulation
Cotton tip applicators Puritan 25-806 10WC Sterile long stemmed cotton tip applicators
Eye Ointment Puralube 17033-211-38 Lubricating petrolatum and mineral oil based ophthalmic ointment
Handwarmers  Hothands  HH2 Air-activated heat packs
Ibuprofen Up & Up 094-01-0245 100 mg per 5 mL in liquid suspension
Isoflurane Henry Schein INC 1182097 Liquid anesthetic for use in anesthetic vaporizer
Scalpels Integra Miltex 4-410 #10 disposable scalpel blade
Suture Covidien SP5686G 45 cm swedged 5-0 monofilament polypropylene suture
Stereotaxic Unit
High Speed Drill (Foredom) Kopf Model 1474 Max of 38,000 RPM
Mouse Gas Anesthesia Head Holder Kopf Model 923-B Mouth bar with teeth hole and nosecone
Non-Rupture Ear Bars Kopf Model 922 Ear bars suitable for mouse applications
Stereotaxic Instrument Kopf Model 940 Base plate, frame and linear scale assembly with digital readout monitor
Anesthesia Machine
SomnoSuite Low-Flow Digital Vaporizer Kent Scientific SS-01 Digital anesthesia machine
SomnoSuite Starter Kit for mice Kent Scientific SOMNO-MSEKIT Includes induction chamber, 2x anesthesia syringes, 18" tubing, plastic nosecone, 2x waste aneshesia gas canisters

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記事を引用
Calvarial Window Generation in Mouse Model: A Surgical Procedure to Create Window in the Top Part of the Mouse Skull. J. Vis. Exp. (Pending Publication), e20609, doi: (2023).

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