Preparation of Formalin-Fixed Paraffin-Embedded Tumor Samples: A Protocol to Preserve Tissue and Cytology Samples from Non-small Cell Lung Cancer (NSCLC)

Published: April 30, 2023

Abstract

Source: Ilié, M., et al. Using 22C3 Anti-PD-L1 Antibody Concentrate on Biopsy and Cytology Samples from Non-small Cell Lung Cancer Patients. J. Vis. Exp. (2018).

This protocol describes the preparation of formalin-fixed paraffin-embedded tumor samples which are used to preserve tissue and cytology samples from non-small cell lung cancer (NSCLC)

Protocol

1. Preparation of Tumor Tissue Samples Fix tumor tissue in 10% neutral buffered formalin (NBF) in a cassette. NOTE: Tumor tissue from proximal lung tumors is generally obtained by bronchoscopy, performed under moderate sedation by a pulmonologist, or lung specialist. For peripheral lesions and diffuse lung disease, a transbronchial or needle biopsy is indicated. These procedures are usually done in a surgery room or intensive care unit. Embed the cassette in paraffin. …

開示

The authors have nothing to disclose.

Materials

NovaPrep HQ1 Novacyt NA Preservative solution for
cytology specimens
Novaprep® HQ+ B Novacyt NA Mucolytic solution
Tissue-Tek VIP 6 Sakura 6042 VIP 6
Tissue-Tek TEC 5 Tissue
Embedding Console System
Sakura 5229 TEC 5
microscope glass slide
SuperFrost Plus
Thermo Fisher Scientific 4951PLUS4
DL-Dithiothreitol powder Sigma-Aldrich D3801
Heidolph Multi Reax Vortex Shaker Thermo Fisher Scientific 13-889-410
Shandon Cytoblock Thermo Fisher Scientific 7401150

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記事を引用
Preparation of Formalin-Fixed Paraffin-Embedded Tumor Samples: A Protocol to Preserve Tissue and Cytology Samples from Non-small Cell Lung Cancer (NSCLC). J. Vis. Exp. (Pending Publication), e20241, doi: (2023).

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