Preparation of Formalin-Fixed Paraffin-Embedded Tumor Samples: A Protocol to Preserve Tissue and Cytology Samples from Non-small Cell Lung Cancer (NSCLC)
Published: April 30, 2023
Abstract
Source: Ilié, M., et al. Using 22C3 Anti-PD-L1 Antibody Concentrate on Biopsy and Cytology Samples from Non-small Cell Lung Cancer Patients. J. Vis. Exp. (2018).
This protocol describes the preparation of formalin-fixed paraffin-embedded tumor samples which are used to preserve tissue and cytology samples from non-small cell lung cancer (NSCLC)
Protocol
1. Preparation of Tumor Tissue Samples Fix tumor tissue in 10% neutral buffered formalin (NBF) in a cassette. NOTE: Tumor tissue from proximal lung tumors is generally obtained by bronchoscopy, performed under moderate sedation by a pulmonologist, or lung specialist. For peripheral lesions and diffuse lung disease, a transbronchial or needle biopsy is indicated. These procedures are usually done in a surgery room or intensive care unit. Embed the cassette in paraffin. …
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The authors have nothing to disclose.
Materials
| NovaPrep HQ1 | Novacyt | NA | Preservative solution for cytology specimens |
| Novaprep® HQ+ B | Novacyt | NA | Mucolytic solution |
| Tissue-Tek VIP 6 | Sakura | 6042 VIP 6 | |
| Tissue-Tek TEC 5 Tissue Embedding Console System |
Sakura | 5229 TEC 5 | |
| microscope glass slide SuperFrost Plus |
Thermo Fisher Scientific | 4951PLUS4 | |
| DL-Dithiothreitol powder | Sigma-Aldrich | D3801 | |
| Heidolph Multi Reax Vortex Shaker | Thermo Fisher Scientific | 13-889-410 | |
| Shandon Cytoblock | Thermo Fisher Scientific | 7401150 |
