In this video we describe a technique of administering a solution of interest into the mammary duct of a rabbit and confirm the delivery by high-resolution ultrasound imaging of contrast agents.
Protocol
1. Preoperative Preparation Record the body weight of each rabbit. As with all preclinical studies, monitor animal weights regularly to assess potential toxicity. Prior to anesthetizing the rabbit, fill a 50 mL conical tube with commercially available ultrasound gel and spin at 500 x g for 30 s; there should be no visible bubbles in the gel upon completion of the centrifugation. Administer glycopyrrolate subcutaneously at a dose of 0.1 mg/kg and acepromazine intramuscularly at a dose of 0….
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The authors have nothing to disclose.
Materials
MicroMarker non-targeted contrast reagent
VisualSonics
VS-11694
Luer Lock 1 mL Syringes
BD
309628
Glycopyrrolate 0.2 mg/mL
Wedgewood Compounding Pharmacy
GLYCOP-INJ013VC
6 month shelf life, supply may be limited.
Ketamine HCL 100 mg/mL
Animal Health International
21250699
http:// www.animalhealthinternational.com/
Acepromazine 10 mg/mL
Animal Health International
17640541
Xylazine 20 mg/mL
Animal Health International
20101547
Hair Removing Cream
Veet
Sensitive skin solution. Available through local retailers.
Rabbit Intraductal Injection: Localized Delivery of Solution of Interest into the Rabbit Mammary Gland. J. Vis. Exp. (Pending Publication), e20224, doi: (2023).