Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices
概要
One feature of Alzheimer’s Disease is the elevation of Aβ1-42 peptide. Here we provide a protocol for preparing synthetic Aβ1-42 oligomers, which impairs hippocampal Long-Term Potentiation, a cellular correlate of memory. This procedure is useful for investigating mechanisms of Aβ-induced pathology and drug screening.
Abstract
Protocol
Resuspending β-amyloid peptide
Before getting started have ready:
- Aβ1-42 (lyophilized powder)
- 1,1,1,3,3,3-Hexafluoro-2-Propanol (HFIP)
- Low-binding polypropylene microcentrifuge tubes (1.5 ml)
- GasTight Hamilton syringe with Teflon plunge stop (250 μl)
- Dimethylsulfoxide (DMSO)
- Allow lyophilized Aβ1-42 to equilibrate at room temperature for 30 minutes to avoid condensation upon opening the peptide vial.
- Under the fume hood, re-suspend Aβ1-42 peptide in ice cold HFIP to obtain a 1 mM solution and vortex the solution for a few seconds.
- Using a glass GasTight Hamilton syringe with Teflon plug, quickly divide the Aβ1-42/HFIP solution equally into three polypropylene vials and seal the vials.
- The vials are then incubated for 2 hours to allow for Aβ monomerization. Next, open the vials and concentrate the Aβ1-42/HFIP solution under vacuum by using a SpeedVac centrifuge (800 g, room temperature) until a clear peptide film is observed at the bottom of the vials. Check carefully the temperature inside the SpeedVac centrifuge to avoid peptide degradation (max 25°C).
- Seal the vials and store the aliquots at -80°C. Aβ film can be stored for 6 months.
- Re-suspend one film by adding DMSO to obtain a concentration up to 5 mM Aβ1-42. Sonicate in the water bath for 10 minutes to ensure complete re-suspension.
- Aliquot this 5mM Aβ1-42 solution into polypropylene vials. Seal all the vials and store them at -20°C. Aliquots should be thawed only once. Beware of water condensation inside the freezer. Peptides in solution degrade very easily.
Oligomerization
Before getting started have ready:
- Aβ1-42/DMSO (5mM)
- Sterile phosphate buffer
- Low-binding polypropylene microcentrifuge tubes (1.5 ml)
- Low-binding polypropylene centrifuge tubes (50 ml)
- Dilute the obtained 5mM Aβ1-42/DMSO aliquot with sterile phosphate buffer (up to 100 μl).
- Vortex for 30 seconds and then incubate for 12 hours at 4°C
Hippocampal slice treatment
When treating hippocampal slices, it is important to avoid non-specific peptide adhesion onto beakers, perfusion tubing surfaces, and the recording chamber. Use preferentially low-binding polypropylene tubing and containers. Avoid the use of glassware or generic plastic-ware. Perfusion media should be serum- or albumin-free.
Aβ perfusion
Before getting started have ready:
- Oligomerized Aβ1-42
- Recording buffer (in mM: 124 NaCl, 4.4 KCl, 1 Na2HPO4, 25 NaHCO3, 2 CaCl2, 2 MgCl2, and 10 glucose)
- Electrophysiological recording set-up with interface chamber
- Transverse hippocampal slices incubated in oxygenated recording buffer for at least 90 minutes post-dissection (T = 29° C; constant perfusion rate = 2 ml/minute)
- Immediately prior to the experiment, dilute the oligomerized Aβ1-42 aliquot to the appropriate working concentration (200 nM or higher) in a polypropylene tube with pre-oxygenated recording buffer.
- Perfuse the Aβ1-42 solution for 20 minutes to the slices before the induction of synaptic plasticity.
Induction of plasticity and follow-up
- Typically, the most widely-used model of synaptic plasticity is the LTP. It can be induced through tetanic stimulation of a given synapse in the hippocampus. We record field responses from synapses between the Shaffer’s collateral projections and CA1 pyramidal neurons in the stratum radiatum. Our tetanic stimulation consists of a theta burst stimulation which includes 3 trains separated by 15 second intervals. Each train consists of 10 bursts at 5 Hz where each burst entails 5 pulses at 100 Hz. Apply the tetanic stimulation immediately after the perfusion of oligomerized Aβ has been completed.
- Immediately after the tetanic stimulation switch back to perfusion with normal recording buffer. Maintain a constant perfusion rate, recording chamber temperature and proper oxygenation throughout the duration of the experiment.
Representative Results and possible problems
The Aβ oligomerized according to the classical protocol of Stine et coll.1, generates Aβ monomers and a variety of oligomers of different sizes (dimer, trimer, etc.) The perfusion of oligomerized Aβ1-42 leads to decreased LTP in Aβ-treated slices compared to control slices. Under our experimental conditions, where 3-5 month old C57BL/6J male mice are used, LTP in 200 nM Aβ-treated slices is on average 150% of the baseline values at two hours after tetanic stimulation, while in control slices LTP values are on average 200-250% of baseline2. In some cases, treatment with Aβ may fail to reduce hippocampal LTP. Several critical errors that might occur include excessive film drying during Aβ concentration and incomplete oligomerization. Aβ perfusion in hippocampal slices might be another source of concern because of the physicochemical properties of the Aβ peptide in solution, which is prone to non-specific adhesion to plastics. Troubleshooting of these issues is discussed below.
Discussion
As described above, several problems in the preparation of oligomeric Aβ may result in unimpaired LTP. One way to evaluate whether Aβ degradation or the lack of Aβ oligomerization may have occurred is to evaluate the Aβ preparation using TRIS-Tricine PAGE/Western Blotting analysis and analytical ultracentrifugation (not described in this protocol). When Western Blotting samples are prepared under non-denaturing/non-reducing conditions, a successful preparation should generate a Western Blotting signature featuring bands corresponding to monomers and different oligomers3. An often occurring issue is the peptide instability during the drying step in the SpeedVac concentrator. In fact, high-temperature drying leads to protein degradation, noticeable by marked peptide coloration to brown. Concentration of the HFIP solution has to be carried out at room temperature and monitored carefully. Given the several procedural steps occurring in the protocol, errors in dilution may accidentally lead to poor oligomerization or misleading Aβ concentration. Interestingly, the perfusion with Aβ concentrations lower than suggested would result in increased LTP values3. Again, perfusion affects the possible outcome of Aβ treatment on slices. Indeed, maintaining a constant temperature of the bath solution in our electrophysiological experiments is critical as temperature is known to affect Aβ conformations when solved in phosphate buffer. Another source of concern is that oligomeric Aβ may non-specifically stick to plastic surfaces of containers and tubing used for preparation and perfusion thereby reducing the effective concentration exposed to hippocampal slices. Thus, it is critical to use low-protein binding plastic-ware to ensure reliable preparations and perfusions throughout different experiments. In this issue, we propose polypropylene plastic-ware which has been shown not to affect the ratio of different Aβ species4. Extensive literature suggests that Alzheimer’s Disease originates as a synaptic disorder5. It is likely that the subtlety and variability of the earliest amnesic symptoms, occurring in the absence of any other clinical signs of brain injury, are due to discrete changes in the function of a single synapse, produced at least in part, by Aβ species (e.g. Aβ42 and Aβ40)2,6,7,8. The discovery that a preparation containing oligomerized synthetic Aβ1-42 can impair LTP9 in vitro has led to numerous investigations into mechanisms of synaptic dysfunction induced by Aβ elevation in brain. Using this protocol, we describe a procedure for preparing oligomeric Aβ which successfully impairs LTP at the Shaffer collateral-stratum radiatum connection in hippocampal slices. This experimental paradigm has tremendous value for investigating mechanisms of Aβ-mediated pathogenesis as well as testing potential drugs, which may mitigate synaptic dysfunction2,10,11.
開示
The authors have nothing to disclose.
Acknowledgements
This work was supported by NIH National Institute of Neurological Disorders and Stroke (NINDS) (NS049442).>
Materials
| Material Name | タイプ | Company | Catalogue Number | Comment |
|---|---|---|---|---|
| Aβ 1-42 (lyophilized) | American Peptide Co. | 62-0-80 | ||
| 1,1,1,3,3,3-Hexafluoro-2-Propanol (HFIP) | Fluka | 52517 | ||
| Dimethylsulfoxide (DMSO) | Biotium | 90082 | ||
| 50mL Polypropylene Centrifuge Tubes | Corning | 05-538-67 | ||
| 1.5ml MaxyClear microtubes Maximum recovery | Axygen | MCT-150-L-C | ||
| Phosphate-Buffered Saline pH 7.4 | Invitrogen-Gibco | 10010-023 | ||
| GASTIGHT Syringes 250 μL | Hamilton | 1725 | ||
| Bath sonicator | Branson | 3510 | ||
| Savant SpeedVac Concentrator System | Various distributors | SC 110A |
参考文献
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