Cell migration is a complex process that requires precise coordination between different cellular components, particularly the cytoskeleton which comprises actin filaments, microtubules, and intermediate filaments. During migration, actin filaments are dynamically reorganized at the cell periphery and help establish the cell’s leading and trailing edges. This polarity directs the centrosome, a microtubule organizing center, to position itself in front of the nucleus and orient the growing microtubules towards the leading edge. The kinesin motors carry various cargo on these microtubule tracks, including exocytic vesicles containing integrins and new membrane components, actin remodeling proteins, and intermediate filaments. The intermediate filaments stabilize and promote maturation of the newly formed focal adhesions. At the trailing edge, the focal adhesions are disassembled, and the dynein motors carry the endocytosed integrins along the microtubules towards the leading edge for recycling. Additionally, the cytoskeletal components are cross-linked to each other, and other organelles via linker proteins. These cross-bridges enable the cytoskeletal components to synchronize their polarity and form a cohesive network for cell migration.