JOVE JOURNAL

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Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

Mengxiao Ma¹, Shreya Goyal², Christopher G. Burd¹, Richard J. Chi²

¹Department of Cell Biology,Yale School of Medicine, ²Department of Biological Sciences,University of North Carolina at Charlotte

Journal (Genetics)

Measuring Microbial Mutation Rates with the Fluctuation Assay

Rok Krašovec¹, Huw Richards^2,3, Guillaume Gomez³, Danna R. Gifford¹, Adrien Mazoyer⁴, Christopher G. Knight³

¹School of Biological Sciences,The University of Manchester, ²School of Science,University of Waikato, ³School of Natural Sciences,The University of Manchester, ⁴Department of Mathematics,Université du Québec à Montréal

Journal (Genetics)

Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR

Antonio Mancarella¹, Francesco A. Procopio¹, Tilmann Achsel², Elisa De Crignis³, Brian T. Foley⁴, Giampietro Corradin⁵, Claudia Bagni², Giuseppe Pantaleo¹, Cecilia Graziosi¹

¹Division of Immunology and Allergy,Lausanne University Hospital, ²Department of Fundamental Neuroscience,University of Lausanne, ³Department of Biochemistry,Erasmus Medical Center, ⁴Theoretical Biology and Biophysics Group,Los Alamos National Laboratories, ⁵Department of Biochemistry,University of Lausanne

Journal (Genetics)

Identification of Circular RNAs using RNA Sequencing

Shobana Sekar^1,2, Philipp Geiger^1,2, Lori Cuyugan^1,2, Annalee Boyle^1,2, Geidy Serrano^2,3, Thomas G. Beach^2,3, Winnie S. Liang^1,2

¹Translational Genomic Research Institute, ²Arizona Alzheimer's Consortium, ³Banner Sun Health Research Institute

Journal (Genetics)

Identification of Host Pathways Targeted by Bacterial Effector Proteins using Yeast Toxicity and Suppressor Screens

Robert Faris¹, Mary M. Weber¹

¹Department of Microbiology and Immunology,University of Iowa

Journal (Genetics)

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

Yassemine Khawajkie¹, Nawel Mechtouf², Phuong Nguyen², Rima Slim^1,2,3

¹Department of Experimental Medicine,McGill University, ²Department of Human Genetics,McGill University, ³Department of Obstetrics and Gynecology,McGill University

Journal (Genetics)

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Shao-Yen Kao*¹, Elena Nikonova*¹, Keshika Ravichandran*¹, Maria L. Spletter^1,2

¹Biomedical Center, Department of Physiological Chemistry,Ludwig-Maximilians-University Munich, ²Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry,Ludwig-Maximilians-University Munich

Journal (Genetics)

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Vivek Bhaskaran¹, Pierpaolo Peruzzi¹

¹Department of Neurosurgery,Brigham and Women's Hospital-Harvard Medical School

Journal (Genetics)

A Murine Cell Line Based Model of Chronic CDK9 Inhibition to Study Widespread Non-Genetic Transcriptional Elongation Defects (TE^deff) in Cancers

Vishnu Modur¹, Navneet Singh¹, Belal Muhammad¹

¹Division of Experimental Hematology and Cancer Biology, Cancer and Blood Diseases Institute,Cincinnati Children's Hospital Medical Center (CCHMC)

Journal (Genetics)

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Fabian L. Cardenas-Diaz*¹, Jean Ann Maguire*¹, Paul Gadue^1,2,3, Deborah L. French^1,2,3

¹Center for Cellular and Molecular Therapeutics,The Children's Hospital of Philadelphia, ²Department of Pathology and Laboratory Medicine,The Children's Hospital of Philadelphia, ³Department of Pathology and Laboratory Medicine,University of Pennsylvania

Journal (Genetics)

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo

Hellen E. Ahrens*¹, Henriette Henze*¹, Svenja C. Schüler¹, Manuel Schmidt¹, Sören S. Hüttner¹, Julia von Maltzahn¹

¹Leibniz-Institute on Aging - Fritz-Lipmann-Institute

Journal (Genetics)

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Mohamed A. Sobhy*¹, Amer Bralić*¹, Vlad-Stefan Raducanu¹, Muhammad Tehseen¹, Yujing Ouyang¹, Masateru Takahashi¹, Fahad Rashid¹, Manal S. Zaher¹, Samir M. Hamdan¹

¹Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division (BESE),King Abdullah University of Science and Technology (KAUST)

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