JOVE JOURNAL

生物学

Showing 337 - 348 in 3,291 videos.

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Journal (Biology)

Flow Cytometric Analysis of Mitophagy in Murine Pancreatic β-Cells Using the Genetically Encoded mt-Keima Reporter

Elena Levi-D’Ancona^1,2, Vaibhav Sidarala¹, Scott A. Soleimanpour^1,3,4

¹Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes,University of Michigan, Ann Arbor, ²Graduate Program in Immunology,University of Michigan Medical School, ³Department of Molecular and Integrative Physiology,University of Michigan, Ann Arbor, ⁴VA Ann Arbor Healthcare System

Journal (Biology)

Complementary Approaches to Interrogate Mitophagy Flux in Pancreatic β-Cells

Elena Levi-D’Ancona^1,2, Vaibhav Sidarala¹, Scott A. Soleimanpour^1,3,4

¹Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes,University of Michigan, Ann Arbor, ²Graduate Program in Immunology,University of Michigan Medical School, ³Department of Molecular and Integrative Physiology,University of Michigan, Ann Arbor, ⁴VA Ann Arbor Healthcare System

Journal (Biology)

Single-Cell Dissociation of Murine Islets for Flow Cytometric Analysis of β-Cell Mitophagy

Elena Levi-D’Ancona^1,2, Vaibhav Sidarala¹, Scott A. Soleimanpour^1,3,4

¹Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes,University of Michigan, Ann Arbor, ²Graduate Program in Immunology,University of Michigan Medical School, ³Department of Molecular and Integrative Physiology,University of Michigan, Ann Arbor, ⁴VA Ann Arbor Healthcare System

Journal (Biology)

Quantifying Fitness Costs in Transgenic Aedes aegypti Mosquitoes

Adeline E. Williams^1,2, Irma Sanchez-Vargas¹, Lindsay E. Martin^2,3, Ines Martin-Martin^2,4, Susi Bennett¹, Ken E. Olson¹, Eric Calvo²

¹Center for Vector-borne Infectious Diseases, Department of Microbiology, Immunology, and Pathology,Colorado State University, ²Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases,National Institutes of Health (NIH), ³Department of Biological Sciences,Vanderbilt University, ⁴National Center for Microbiology,Instituto de Salud Carlos III

Journal (Biology)

Inoculation of Maize Leaf Sheaths with Pathogenic Fungi for Assessing Fungal Pathogenicity

Renata Belisário¹, Maria F. Torres^1,2, Ester A. S. Buiate^1,3, Katia V. Xavier^1,4, Etta M. Nuckles¹, Lisa J. Vaillancourt¹

¹Department of Plant Pathology,University of Kentucky, ²Department of Biological Sciences,University of Cincinnati, ³Bayer Crop Science, ⁴Everglades Research and Education Center,University of Florida

Journal (Biology)

Preparation of Fungal Infected Detached Maize Leaf Sheaths for Live-Cell Imaging

Renata Belisário¹, Maria F. Torres^1,2, Ester A. S. Buiate^1,3, Katia V. Xavier^1,4, Etta M. Nuckles¹, Lisa J. Vaillancourt¹

¹Department of Plant Pathology,University of Kentucky, ²Department of Biological Sciences,University of Cincinnati, ³Bayer Crop Science, ⁴Everglades Research and Education Center,University of Florida

Journal (Biology)

Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens

Renata Belisário¹, Maria F. Torres^1,2, Ester A. S. Buiate^1,3, Katia V. Xavier^1,4, Etta M. Nuckles¹, Lisa J. Vaillancourt¹

¹Department of Plant Pathology,University of Kentucky, ²Department of Biological Sciences,University of Cincinnati, ³Bayer Crop Science, ⁴Everglades Research and Education Center,University of Florida

Journal (Biology)

Dissection and Removal of the Temporal Bone to Collect Auditory Epithelia from Newborn Mice

Kun-Yi Wu*¹, Bo-Tao Wang*², Yun-Jia He², Jing-Yi Xie², Zi-Chen Chen², Ying Gao²

¹Department of Core Research Laboratory,The Second Affiliated Hospital of Xi’an Jiaotong University, ²Department of Otolaryngology,The Second Affiliated Hospital of Xi’an Jiaotong University

Journal (Biology)

Enzymatic Disaggregation of the Auditory Epithelium to Obtain Auditory Hair Cells

Kun-Yi Wu*¹, Bo-Tao Wang*², Yun-Jia He², Jing-Yi Xie², Zi-Chen Chen², Ying Gao²

¹Department of Core Research Laboratory,The Second Affiliated Hospital of Xi’an Jiaotong University, ²Department of Otolaryngology,The Second Affiliated Hospital of Xi’an Jiaotong University

Journal (Biology)

Isolation and Culture of Primary Cochlear Hair Cells from Neonatal Mice

Kun-Yi Wu*¹, Bo-Tao Wang*², Yun-Jia He², Jing-Yi Xie², Zi-Chen Chen², Ying Gao²

¹Department of Core Research Laboratory,The Second Affiliated Hospital of Xi’an Jiaotong University, ²Department of Otolaryngology,The Second Affiliated Hospital of Xi’an Jiaotong University

Journal (Biology)

Preparation of FFPE DNA and RNA Samples for Sequencing Using an Automated Purification Instrument (API)

Christophe Bontoux^1,2,3,4,5, Virginie Lespinet-Fabre^1,2,3,4, Olivier Bordone^1,2,3,4, Virginie Tanga^1,2,3,4, Maryline Allegra^1,2,3,4, Myriam Salah^1,2,3,4, Salomé Lalvée^1,2,3,4, Samantha Goffinet^1,2,3,4, Jonathan Benzaquen^3,4,5,6, Charles-Hugo Marquette^3,4,5,6, Marius Ilié^1,2,3,4,5, Véronique Hofman^1,2,3,4,5, Paul Hofman^1,2,3,4,5

¹Laboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice,Université Côte d’Azur, CHU de Nice, ²Hospital-Integrated Biobank (BB-0033-00025),Université Côte d'Azur, Hôpital Pasteur, CHU de Nice, ³Institut Hospitalo-Universitaire (IHU), RespirERA,Université Côte d'Azur, Hôpital Pasteur, CHU de Nice, ⁴FHU OncoAge,Université Côte d'Azur, ⁵Team 4, Institute of Research on Cancer and Aging (IRCAN), CNRS INSERM, Centre Antoine-Lacassagne,Université Côte d'Azur, ⁶Department of Pulmonary Medicine and Thoracic Oncology, Centre Hospitalier Universitaire de Nice,Université Côte d'Azur

Journal (Biology)

Ultra-Fast Amplicon-Based Next-Generation Sequencing in Non-Squamous Non-Small Cell Lung Cancer

Christophe Bontoux^1,2,3,4,5, Virginie Lespinet-Fabre^1,2,3,4, Olivier Bordone^1,2,3,4, Virginie Tanga^1,2,3,4, Maryline Allegra^1,2,3,4, Myriam Salah^1,2,3,4, Salomé Lalvée^1,2,3,4, Samantha Goffinet^1,2,3,4, Jonathan Benzaquen^3,4,5,6, Charles-Hugo Marquette^3,4,5,6, Marius Ilié^1,2,3,4,5, Véronique Hofman^1,2,3,4,5, Paul Hofman^1,2,3,4,5

¹Laboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice,Université Côte d’Azur, CHU de Nice, ²Hospital-Integrated Biobank (BB-0033-00025),Université Côte d'Azur, Hôpital Pasteur, CHU de Nice, ³Institut Hospitalo-Universitaire (IHU), RespirERA,Université Côte d'Azur, Hôpital Pasteur, CHU de Nice, ⁴FHU OncoAge,Université Côte d'Azur, ⁵Team 4, Institute of Research on Cancer and Aging (IRCAN), CNRS INSERM, Centre Antoine-Lacassagne,Université Côte d'Azur, ⁶Department of Pulmonary Medicine and Thoracic Oncology, Centre Hospitalier Universitaire de Nice,Université Côte d'Azur

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