JOVE JOURNAL

生化学

Showing 601 - 612 in 778 videos.

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Journal (Biochemistry)

Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy

Andreas Reif¹, Anass Chiki¹, Jonathan Ricci¹, Hilal A. Lashuel¹

¹Laboratory of Molecular and Chemical Biology of Neurodegeneration,Ecole Polytechnique Fédérale de Lausanne (EPFL)

Journal (Biochemistry)

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H⁺

Ya-Min Du¹, Shen-Li Zheng¹, Li Liu¹, Josef Voglmeir¹, Gabriel Yedid²

¹Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology,Nanjing Agricultural University, ²College of Life Science,Nanjing Agricultural University

Journal (Biochemistry)

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

Anita Carija¹, Salvador Ventura¹, Susanna Navarro¹

¹Institut de Biotecnologia i Biomedicina and Departament de Bioquímica i Biologia Molecular,Universitat Autònoma de Barcelona

Journal (Biochemistry)

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Dana M. Freund¹, Katherine A. Sammons², Nokwanda P. Makunga³, Jerry D. Cohen¹, Adrian D. Hegeman²

¹Department of Horticultural Science, Microbial and Plant Genomics Institute,University of Minnesota, ²Department of Horticultural Science, Department of Plant and Microbial Biology, Microbial and Plant Genomics Institute,University of Minnesota, ³Department of Botany and Zoology,Stellenbosch University

Journal (Biochemistry)

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue

David Jelinek^1,2, Aimee Flores^1,3, Melanie Uebelhoer¹, Vincent Pasque², Kathrin Plath^2,3, M. Luisa Iruela-Arispe^1,3, Heather R. Christofk^2,3, William E. Lowry^1,3, Hilary A. Coller^1,2,3

¹Department of Molecular, Cell and Developmental Biology,UCLA, ²Department of Biological Chemistry,David Geffen School of Medicine, ³Molecular Biology Institute Interdepartmental Program,UCLA

Journal (Biochemistry)

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus

Dominik Schumacher¹, Lotte Søgaard-Andersen¹

¹Department of Ecophysiology,Max Planck Institute for Terrestrial Microbiology

Journal (Biochemistry)

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Kang-Yi Su*^1,2, Steven D. Goodman*³, Hung-Ming Lai¹, Rong-Syuan Yen¹, Wei-Yao Hu¹, Wern-Cherng Cheng², Liang-In Lin^1,2, Ya-Chien Yang^1,2, Woei-Horng Fang^1,2

¹Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine,National Taiwan University, ²Department of Laboratory Medicine,National Taiwan University Hospital, ³Center for Microbial Pathogenesis, Nationwide Children's Hospital and the Department of Pediatrics,The Ohio State University

Journal (Biochemistry)

Defining Hsp33’s Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Rosi Fassler*¹, Nufar Edinger*¹, Oded Rimon¹, Dana Reichmann¹

¹Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, Safra Campus Givat Ram,The Hebrew University of Jerusalem

Journal (Biochemistry)

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Tabitha A. Peterson¹, Mark A. Stamnes¹, Robert C. Piper¹

¹Molecular Physiology and Biophysics,University of Iowa

Journal (Biochemistry)

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

Cuiping Li¹, Weiyi Lai¹, Hailin Wang¹

¹State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences

Journal (Biochemistry)

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

Hiroyuki Yamamoto¹, Yoshikazu Sawaguchi², Michio Kimura¹

¹Department of Microbiology and Molecular Cell Biology,Nihon Pharmaceutical University, ²Department of Clinical Pharmaceutics,Nihon Pharmaceutical University

Journal (Biochemistry)

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

Huang Chunhui^1,2, Xu Dilin¹, Zhang Ke¹, Shentu Jieyi², Yan Sicheng¹, Wu Dapeng³, Wang Qinwen¹, Cui Wei^1,2

¹Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine,Ningbo University, ²Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center,Ningbo University, ³School of Materials Science and Chemical Engineering,Ningbo University

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