JOVE JOURNAL

生化学

Showing 529 - 540 in 778 videos.

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Hanan B. Elajaili¹, Laura Hernandez-Lagunas¹, Kalina Ranguelova², Sergey Dikalov³, Eva Nozik-Grayck¹

¹Cardiovascular Pulmonary Research Laboratories and Pediatric Critical Care Medicine, Department of Pediatrics,University of Colorado Anschutz Medical Campus, ²Bruker BioSpin Corp, ³Department of Medicine, Division of Clinical Pharmacology,Vanderbilt University Medical Center

Journal (Biochemistry)

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

Javier A. Jaimes*^1,2, Jean K. Millet*^1,3, Monty E. Goldstein^1,4, Gary R. Whittaker¹, Marco R. Straus¹

¹Department of Microbiology and Immunology, College of Veterinary Medicine,Cornell University, ²Department of Microbiology, College of Agricultural and Life Sciences,Cornell University, ³Virologie et Immunologie Moléculaires, Domaine de Vilvert,INRA, ⁴Department of Cell Biology and Molecular Genetics,University of Maryland

Journal (Biochemistry)

Identification of Functional Protein Regions Through Chimeric Protein Construction

Juan M. Adrian-Segarra¹, Holger Lörchner^1,2, Thomas Braun^1,2, Jochen Pöling^1,2

¹Department of Cardiac Development and Remodelling,Max Planck Institute for Heart and Lung Research, ²Partner site Rhein-Main,German Centre for Cardiovascular Research (DZHK)

Journal (Biochemistry)

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Bushra Husain¹, Sairupa Paduchuri¹, Sree R. Ramani², Nadia Martinez-Martin¹

¹Receptor Discovery group, Microchemistry, Proteomics and Lipidomics Department,Genentech, ²Portfolio Management and Operations,Genentech

Journal (Biochemistry)

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

Magali Court^1,2, Marie Malier^1,2, Arnaud Millet^1,2,3

¹Team Mechanobiology, Immunity and Cancer, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, ²Université Grenoble Alpes, ³Pôle Recherche,Centre Hospitalier Universitaire des Alpes

Journal (Biochemistry)

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Andreas Weber¹, Julia Christin Wehmeyer¹, Vera Schmidt¹, Artur Lichtenberg¹, Payam Akhyari¹

¹Department of Cardiovascular Surgery, Medical Faculty,Heinrich-Heine-University

Journal (Biochemistry)

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells

Vanessa Chen¹, Malte Renz¹

¹Gynecologic Oncology Division,Stanford University School of Medicine

Journal (Biochemistry)

Extraction and Analysis of Taiwanese Green Propolis

Chun-Ting Chen¹, Yi-Hsuan Chien¹, Yu-Hsiang Yu¹, Yue-Wen Chen¹

¹Department of Biotechnology and Animal Science,National Ilan University

Journal (Biochemistry)

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Emery Haley*¹, Wooyoung Choi*¹, Chen Fan¹, Weinan Sun^2,3, Juan Du¹, Wei Lü¹

¹Van Andel Institute, ²Vollum Institute, ³Janelia Research Campus

Journal (Biochemistry)

Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments

Louisa Jutta Dietz¹, Anuroop Venkateswaran Venkatasubramani¹, Annika Müller-Eigner², Martin Hrabe de Angelis^3,4,5, Axel Imhof¹, Lore Becker³, Shahaf Peleg^2,6,7

¹Munich Center of Integrated Protein Science and Biomedical Center,Ludwig-Maximilians University of Munich, ²Laboratory for Metabolism and Epigenetics in Aging,Leibniz Institute for Farm Animal Biology (FBN), ³German Mouse Clinic, Helmholtz Zentrum Munich,German Research Center for Environment and Health (GmbH), ⁴German Center for Diabetes Research (DZD), ⁵Chair of Experimental Genetics, School of Life Science Weihenstephan,Technische Universität München, ⁶Laboratory for Metabolism and Epigenetics in Brain Aging,Institute of Neuroregeneration & Neurorehabilitation of Qingdao University, ⁷Molecular Biology Division, Biomedical Center, Faculty of Medicine,Ludwig-Maximilians University of Munich

Journal (Biochemistry)

Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

Jae Gon Kim*¹, Sang Woong Park*², Kyung Chul Shin¹, Bokyung Kim¹, Doyoung Byun³, Young Min Bae¹

¹Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science,Konkuk University School of Medicine, ²Department of Emergency Medical Services,Eulji University, ³機械工学科,Sungkyunkwan University

Journal (Biochemistry)

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Aleksandra Skrajna¹, Xiao-Cui Yang¹, Zbigniew Dominski^1,2

¹Integrative Program for Biological and Genome Sciences,University of North Carolina at Chapel Hill, ²Department of Biochemistry and Biophysics,University of North Carolina at Chapel Hill

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