JOVE JOURNAL

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In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

Florian Stritzke^1,2, Hendrik Poeck^1,2,3,4, Simon Heidegger^1,2

¹Department of Medicine III, School of Medicine,Technical University of Munich, ²Center for Translational Cancer Research (TranslaTUM), School of Medicine,Technical University of Munich, ³Department of Internal Medicine III,University Hospital Regensburg, ⁴National Centre for Tumor Diseases WERA

Journal (Cancer Research)

Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product

Ana Marie Landin¹, Cheryl Cox¹, Bin Yu², Nelli Bejanyan^1,2, Marco Davila^1,2, Linda Kelley^1,2

¹Cell Therapy Facility,Moffitt Cancer Center and Research Institute, ²Department of Immunology,Moffitt Cancer Center and Research Institute

Journal (Cancer Research)

An Ex Vivo Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth

Lorela Ciraku¹, Rebecca A. Moeller¹, Emily M. Esquea¹, Wiktoria A. Gocal¹, Edward J. Hartsough^2,4, Nicole L. Simone^3,4, Joshua G. Jackson², Mauricio J. Reginato^1,4

¹生化学・分子生物学科,Drexel University College of Medicine, ²Department of Pharmacology and Physiology,Drexel University College of Medicine, ³放射線腫瘍学科,Thomas Jefferson University, ⁴Sidney Kimmel Cancer Center,Thomas Jefferson University

Journal (Cancer Research)

Three Differential Expression Analysis Methods for RNA Sequencing: limma, EdgeR, DESeq2

Shiyi Liu*¹, Zitao Wang*¹, Ronghui Zhu¹, Feiyan Wang², Yanxiang Cheng¹, Yeqiang Liu²

¹Department of Obstetrics and Gynecology,Renmin Hospital of Wuhan University, ²Department of Pathology, Shanghai Skin Disease Hospital,Tongji University School of Medicine

Journal (Cancer Research)

Analysis of Lymph Node Volume by Ultra-High-Frequency Ultrasound Imaging in the Braf/Pten Genetically Engineered Mouse Model of Melanoma

Marianna Vitiello^1,2, Claudia Kusmic¹, Francesco Faita¹, Laura Poliseno^1,2

¹Institute of Clinical Physiology,National Research Council (IFC-CNR), ²Oncogenomics Unit,Core Research Laboratory, ISPRO

Journal (Cancer Research)

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

Hiroki Kobayashi^1,2,3,4, Krystyna A. Gieniec^1,2, Jia Q. Ng^1,2, Jarrad Goyne^1,2, Tamsin R. M. Lannagan^1,2, Elaine M. Thomas^1,2, Georgette Radford^1,2, Tongtong Wang^1,2, Nobumi Suzuki^1,2,5, Mari Ichinose^1,2, Josephine A. Wright², Laura Vrbanac^1,2, Alastair D. Burt⁶, Masahide Takahashi^3,4,7, Atsushi Enomoto³, Daniel L. Worthley², Susan L. Woods^1,2

¹Adelaide Medical School,University of Adelaide, ²South Australian Health and Medical Research Institute (SAHMRI), ³病理学科,Nagoya University Graduate School of Medicine, ⁴Division of Molecular Pathology, Center for Neurological Disease and Cancer,Nagoya University Graduate School of Medicine, ⁵Department of Gastroenterology, Graduate School of Medicine,The University of Tokyo, ⁶Translational and Clinical Research Institute,Newcastle University, ⁷International Center for Cell and Gene Therapy,Fujita Health University

Journal (Cancer Research)

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Rivak Punchoo^1,2, Esther Zhou¹, Sachin Bhoora¹

¹Department of Chemical Pathology, Faculty of Health Sciences,University of Pretoria, ²Tshwane Academic Division,National Health Laboratory Service

Journal (Cancer Research)

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

Jody Fromm Longo¹, Stephanie N. Brosius^2,3, Steven L. Carroll¹

¹Department of Pathology and Laboratory Medicine,Medical University of South Carolina, ²Department of Pediatrics and Neurology,Perelman School of Medicine of the University of Pennsylvania, ³Division of Child Neurology,Children's Hospital of Philadelphia

Journal (Cancer Research)

Sentinel Lymph Node Mapping and Biopsy for Endometrial Cancer at Early Stage with Laparoscopy

Bo Wang¹, Yu Xue¹, Qian Wang¹, Yan Xu¹, Xiaojun Chen¹, Chao Wang¹

¹Obstetrics and Gynecology Hospital of Fudan University

Journal (Cancer Research)

Robot Assisted Distal Pancreatectomy with Celiac Axis Resection (DP-CAR) for Pancreatic Cancer: Surgical Planning and Technique

Pavan Rao¹, Carl R. Schmidt¹, Brian A. Boone^1,2

¹Division of Surgical Oncology, Department of Surgery,West Virginia University, ²Department of Microbiology, Immunology and Cell Biology,West Virginia University

Journal (Cancer Research)

Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates

Elizabeth R. M. Zunica^1,2,3, Christopher L. Axelrod¹, L. Anne Gilmore^2,4, John P. Kirwan^1,3

¹Integrated Physiology and Molecular Medicine Laboratory,Pennington Biomedical Research Center, ²Clinical Oncology and Metabolism,Pennington Biomedical Research Center, ³Department of Nutrition,Case Western Reserve University, ⁴Department of Clinical Nutrition,University of Texas Southwestern Medical Center

Journal (Cancer Research)

Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research

Giovanna Azzarito¹, Marta Ewa Szutkowska¹, Annalisa Saltari², Edwin K. Jackson³, Brigitte Leeners¹, Marinella Rosselli¹, Raghvendra K. Dubey^1,3

¹Department of Reproductive Endocrinology, Wagistrasse 14,University of Zurich and University Hospital Zurich, ²Department of Dermatology, Wagistrasse 18,University of Zurich and University Hospital Zurich, ³Department of Pharmacology and Chemical Biology,University of Pittsburgh

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