ジャーナル
High-Resolution 3D Reconstruction of Human Brain with SHORT and LSFM
January 26, 2024
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概要

The present protocol provides a step-by-step procedure for rapid and simultaneous optical clearing, muti-round labeling, and 3D volumetric reconstruction of tens of postmortem human brain sections by combining the (SWITCH – H2O2 – Antigen Retrieval – 2,2′-thiodiethanol [TDE]) SHORT tissue transformation technique with light-sheet fluorescence microscopy imaging in a routinely high-throughput protocol.