ジャーナル
生物学
A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

August 18, 2023

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

02:47 min
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Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV)

03:08 min
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Sequencing, Live and Offline Basecalling, Analysis, and Interpretation of Rabies Virus (RABV) Genome

05:00 min
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1School of Biodiversity, One Health & Veterinary Medicine,University of Glasgow, 2Research Institute for Tropical Medicine, 3University of Nairobi, Institute of Tropical and Infectious Diseases, 4Tanzania Industrial Research Development Organization, 5Ifakara Health Institute, 6MRC-University of Glasgow Centre for Virus Research, 7Department of Veterinary Public health,Ahmadu Bello University, 8African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology,Ahmadu Bello University, 9University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis,University of Nairobi

概要

Here, we present a rapid and cost-effective workflow for characterizing rabies virus (RABV) genomes using nanopore technology. The workflow is intended to support genomics-informed surveillance at a local level, providing information on circulating RABV lineages and their placement within regional phylogenies to guide rabies control measures.

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