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ソックス- 2の強制発現によってMEFSからiPS細胞を生成し、OCT - 4、c - Mycの、とKLF4
JoVE Journal
Biologia
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JoVE Journal Biologia
Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4
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ソックス- 2の強制発現によってMEFSからiPS細胞を生成し、OCT - 4、c - Mycの、とKLF4

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13:02 min

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April 07, 2008

DOI:

10.3791/734-v

DOI:
10.3791/734-v

•

13:02 min
April 07, 2008

•

13 Views
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1Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology

Summary

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このビデオでは、誘導レンチウイルスを用いて誘導多能性幹細胞を生成するための手順を示して発現するOct4は、レトロウイルスベクター、c - MycおよびKLF4。

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Cite this Article

Welstead, G. G., Brambrink, T., Jaenisch, R. Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4. J. Vis. Exp. (14), e734, doi:10.3791/734 (2008).

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