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Isolation of Hypothalamic Microglia from Mice Using MACS for In Vitro Assays
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Author Spotlight: Investigating the Impact of Nutrition on Mouse Brain Function and Metabolic Disorders
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Neuroscienze
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JoVE Journal Neuroscienze
Isolation of Hypothalamic Microglia from Freshly Perfused Adult Mouse Brain by Magnetic-Activated Cell Sorting

Author Spotlight: Investigating the Impact of Nutrition on Mouse Brain Function and Metabolic Disorders

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01:34 min

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September 06, 2024

DOI:

10.3791/66769-v

DOI:
10.3791/66769-v

•

01:34 min
September 06, 2024

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21 Views
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1University of Bordeaux, INSERM, Neurocentre Magendie

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Hypothalamic MicrogliaMagnetic-activated Cell SortingBrain HomeostasisNeuronal CircuitsNeurodevelopmentSynaptic PlasticityMicroglia IsolationCD11b+ MicrobeadsCell ViabilityAdult Mouse BrainMicroglial CharacterizationLow YieldBrain Structures

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Kyriakidou, E., Cutugno, G., Allard, C., Nadjar, A. Isolation of Hypothalamic Microglia from Freshly Perfused Adult Mouse Brain by Magnetic-Activated Cell Sorting . J. Vis. Exp. (211), e66769, doi:10.3791/66769 (2024).

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