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Whole Neonatal Cochlear Explants as an In vitro Model
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JoVE Journal Neuroscienze
Whole Neonatal Cochlear Explants as an In vitro Model

Whole Neonatal Cochlear Explants as an In vitro Model

DOI:
10.3791/65160-v

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07:12 min

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July 28, 2023

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, , ,
1Department of Biomedicine,University of Basel Hospital, 2Department of Otorhinolaryngology, Head and Neck Surgery,University of Basel Hospital

Capitoli

  • 00:04Introduction
  • 01:13Isolation of Cochlea, Corti, and Cochlear Explants
  • 03:31Cochlear Explants Culturing
  • 05:04Results: Identification of Corti and Cochlear Explants
  • 06:30Conclusion

Summary

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The current protocol updates previous protocols and incorporates relatively straightforward approaches for culturing high-quality cochlear explants. This provides reliable data acquisition and high-resolution imaging in live and fixed cells. This protocol supports the ongoing trend of studying inner ear cells.

Tags

Keywords: Neonatal Cochlear ExplantIn Vitro ModelSensorineural Hearing LossOtotoxic ModelStructural AnalysisSignaling PathwaysDrug ScreeningEmbryonic DevelopmentCochlear DissectionCochleaSpiral GanglionSpiral LigamentStria VascularisReissner’s Membrane

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