The current protocol updates previous protocols and incorporates relatively straightforward approaches for culturing high-quality cochlear explants. This provides reliable data acquisition and high-resolution imaging in live and fixed cells. This protocol supports the ongoing trend of studying inner ear cells.
Levano, S., Lu, Y., Cortada, M., Bodmer, D. Whole Neonatal Cochlear Explants as an In vitro Model. J. Vis. Exp. (197), e65160, doi:10.3791/65160 (2023).