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Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
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Biologia
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JoVE Journal Biologia
Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

DOI:
10.3791/65135-v

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06:53 min

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May 05, 2023

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, ,
1LabPlaM – Mycoheterotrophic Plants Research Lab, Department of Plant Biology,State University of Campinas (UNICAMP)

Capitoli

  • 00:00Introduction
  • 00:21Isolation of Endophytic Fungi Associated with Plant Organs
  • 03:12Preservation of Purified Fungal Isolates
  • 04:01Micromorphological Characterization (Hyphal Morphology) of Filamentous Fungi
  • 05:10Results: Isolation and Macromorphology Evaluation of Endophytic Fungi Associated with Mycoheterotrophic Plants
  • 06:33Conclusion

Summary

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Traduzione automatica

The current article aims to provide detailed and adequate protocols for the isolation of plant-associated endophytic fungi, long-term preservation of isolates, morphological characterization, and total DNA extraction for subsequent molecular identification and metagenomic analyses.

Tags

Keywords: Endophytic FungiMycoheterotrophic PlantsFungal IsolationFungal CharacterizationTotal DNA ExtractionPDA Culture MediumAntibiotic SupplementationSuperficial DisinfectionColony PurificationAA MediumFungal IdentificationFungal Subculturing

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