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Isolation and Characterization of the Murine Uterosacral Ligaments and Pelvic Floor Organs
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JoVE Journal Biologia
Isolation and Characterization of the Murine Uterosacral Ligaments and Pelvic Floor Organs

Isolation and Characterization of the Murine Uterosacral Ligaments and Pelvic Floor Organs

DOI:
10.3791/65074-v

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05:47 min

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March 03, 2023

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, , , , , ,
1Biomedical Engineering Program,University of Colorado Boulder, 2Paul M. Rady Department of Mechanical Engineering,University of Colorado Boulder, 3Department of Obstetrics and Gynecology,University of Colorado Anschutz Medical Campus, 4BioFrontiers Institute,University of Colorado Boulder, 5Department of Mechanical Science and Engineering,University of Illinois Urbana-Champaign

Capitoli

  • 00:04Introduction
  • 00:57Animal Preparation
  • 01:41Harvesting the Uterosacral Ligament
  • 02:39Bladder, Rectum, and Cervix‐Vagina Harvest
  • 03:53Results: Uterosacral Ligament Characterization
  • 05:02Conclusion

Summary

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This article presents a detailed protocol for dissecting uterosacral ligaments and other pelvic floor tissues, including the cervix, rectum, and bladder in mice, to expand the study of female reproductive tissues.

Tags

Keyword Extraction: Uterosacral LigamentPelvic Floor OrgansPelvic Organ Prolapse (POP)Preclinical ModelWomen’s Health ResearchDissectionAnatomical AttachmentsCervixUretersBladderColonPBSForcepsSutureMechanical TestingImaging

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