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El colgajo axial superficial de la arteria epigástrica inferior para estudiar los efectos del preacondicionamiento isquémico en un modelo de rata
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
The Superficial Inferior Epigastric Artery Axial Flap to Study Ischemic Preconditioning Effects in a Rat Model
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El colgajo axial superficial de la arteria epigástrica inferior para estudiar los efectos del preacondicionamiento isquémico en un modelo de rata

1,279 Views

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09:07 min

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January 27, 2023

DOI:

10.3791/64980-v

DOI:
10.3791/64980-v

•

09:07 min
January 27, 2023

•

5 Views
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1Vascularized Composite Allotransplantation Laboratory,Massachusetts General Hospital, 2Harvard Medical School, 3Department of Plastic, Reconstructive and Aesthetic Surgery, Rennes University Hospital Center (CHU de Rennes),University of Rennes 1, 4Plastic Surgery Research Laboratory,Massachusetts General Hospital, 5Shriners Children’s Boston, 6Center for Engineering in Medicine and Surgery,Massachusetts General Hospital, 7Department of Plastic Surgery and Hand Surgery,University Medical Center Utrecht, 8Department of Plastic, Reconstructive, and Aesthetic Surgery, Groupe Almaviva Santé,Clinique de l’Alma

Summary

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Este protocolo describe la recolección, sutura y monitoreo de colgajos fasciocutáneos en ratas que permiten una buena visualización y manipulación del flujo sanguíneo a través de los vasos epigástricos inferiores superficiales mediante el pinzamiento y la ligadura de los vasos femorales. Esto es crítico para los estudios que involucran preacondicionamiento isquémico.

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Berkane, Y., Alana Shamlou, A., Reyes, J., Lancia, H. H., Filz von Reiterdank, I., Bertheuil, N., Uygun, B. E., Uygun, K., Austen Jr., W. G., Cetrulo Jr., C. L., Randolph, M. A., Lellouch, A. G. The Superficial Inferior Epigastric Artery Axial Flap to Study Ischemic Preconditioning Effects in a Rat Model. J. Vis. Exp. (191), e64980, doi:10.3791/64980 (2023).

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