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刺胞动物模型系统中的染色质免疫沉淀 Exaiptasia diaphana
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11:48 min
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March 17, 2023
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1Red Sea Research Center (RSRC), Biological and Environmental Sciences and Engineering Division (BESE),King Abdullah University of Science and Technology (KAUST), 2KAUST Environmental Epigenetics Program (KEEP), Biological and Environmental Sciences and Engineering Division (BESE),King Abdullah University of Science and Technology (KAUST)
Summary
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介绍了针对模式生物 Exaiptasia diaphana 的组蛋白标记 H3K4me3 的染色质免疫沉淀 （X-ChIP） 测定。该检测的特异性和有效性通过定量PCR和下一代测序得到证实。该协议可以增加对海葵 E. diaphana中蛋白质-DNA相互作用的研究。
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Dix, M. F., Liu, P., Cui, G., Della Valle, F., Orlando, V., Aranda, M. Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana. J. Vis. Exp. (193), e64817, doi:10.3791/64817 (2023).
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