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Immunology and Infection

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JoVE Journal Immunology and Infection
Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique
 

Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique

Article DOI: 10.3791/64671-v • 09:37 min • September 23rd, 2022
September 23rd, 2022 •
*1,2, *1,2,3, 1,2, 1,2, 1,2, 4, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd
* These authors contributed equally

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The present protocol combines ex vivo stimulation and flow cytometry to analyze polyfunctional T cell (TPF) profiles in peripheral blood mononuclear cells (PBMCs) within Japanese encephalitis virus (JEV)-vaccinated children. The detection method and flow cytometry color scheme of JEV-specific TPFs were tested to provide a reference for similar studies.

Tags

Polyfunctional T Cells Japanese Encephalitis Vaccine Flow Cytometry Technique Detection Method TPFS PBMCs JEV Stimulation Group Control Group Monoclonal Antibodies CD28 CD49d CD107a GolgiPlug Monensin Cell Suspension Microcentrifuge Tube Viability Dye Cell Surface Marker Staining
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