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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
JoVE Journal
Bioingegneria
This content is Free Access.
JoVE Journal Bioingegneria
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

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10:44 min

May 05, 2023

DOI:

10:44 min
May 05, 2023

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Summary

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Here, we present a protocol for the in vitro selection of engineered transcriptional repressors (ETRs) with high, long-term, stable, on-target silencing efficiency and low genome-wide, off-target activity. This workflow allows for reducing an initial, complex repertoire of candidate ETRs to a short list, suitable for further evaluation in therapeutically relevant settings.

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