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Akış Sitometrisine Dayalı Miyokard B Hücrelerinin Nicelleştirilmesi ve Analizi
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells
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Akış Sitometrisine Dayalı Miyokard B Hücrelerinin Nicelleştirilmesi ve Analizi

2,173 Views

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12:46 min

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August 17, 2022

DOI:

10.3791/64344-v

DOI:
10.3791/64344-v

•

12:46 min
August 17, 2022

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8 Views
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1Division of Cardiology, Department of Medicine,The Johns Hopkins University School of Medicine

Tags

Flow CytometryMyocardial B-cellsPerfusionDigestionIsolationAntibody PanelImmune Cell TypesHeartLungLiverB220 AntibodyEuthanasiaHBSSMinced HeartEnzymesDNase ICollagenase IIHyaluronidase

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Cite this Article

Bermea, K. C., Rousseau, S. T., Adamo, L. Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells. J. Vis. Exp. (186), e64344, doi:10.3791/64344 (2022).

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