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Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
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Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
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07:17 min
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December 16, 2022
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Here, we describe a simple technique intended for the efficient generation of genetically modified mice called CRISPR RNP Electroporation of Zygotes (CRISPR-EZ). This method delivers editing reagents by electroporation into embryos at an efficiency approaching 100%. This protocol is effective for point mutations, small genomic insertions, and deletions in mammalian embryos.
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Diallo, C. K., Modzelewski, A. J. Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes. J. Vis. Exp. (190), e64302, doi:10.3791/64302 (2022).
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