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Transfección eficiente del ARNm transcrito in vitro en células cultivadas utilizando nanopartículas de péptido-poloxamina
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
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Transfección eficiente del ARNm transcrito in vitro en células cultivadas utilizando nanopartículas de péptido-poloxamina

2,886 Views

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10:16 min

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August 17, 2022

DOI:

10.3791/64288-v

DOI:
10.3791/64288-v

•

10:16 min
August 17, 2022

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8 Views
, , , , , , , , , ,
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy,Third Military Medical University, 2Department of Pediatrics,Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital,Third Military Medical University

Summary

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Se desarrolla una nanopartícula de péptido-poloxamina autoensamblada (PP-sNp) utilizando un dispositivo de mezcla microfluídica para encapsular y entregar ARN mensajero transcrito in vitro . El ARNm/PP-sNp descrito podría transfectar eficientemente células cultivadas in vitro.

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Cite this Article

Xiao, Q., Liu, Y., Zhang, D., Li, C., Yang, Q., Lu, D., Zhang, W., Rosenecker, J., Zou, Q., Li, Y., Guan, S. Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles. J. Vis. Exp. (186), e64288, doi:10.3791/64288 (2022).

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