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Efficiënte transfectie van in vitro getranscribeerd mRNA in gekweekte cellen met behulp van peptide-poloxamine nanodeeltjes
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
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Efficiënte transfectie van in vitro getranscribeerd mRNA in gekweekte cellen met behulp van peptide-poloxamine nanodeeltjes

2,886 Views

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10:16 min

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August 17, 2022

DOI:

10.3791/64288-v

DOI:
10.3791/64288-v

•

10:16 min
August 17, 2022

•

8 Views
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1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy,Third Military Medical University, 2Department of Pediatrics,Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital,Third Military Medical University

Summary

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Een zelfgeassembleerd peptide-poloxamine nanodeeltje (PP-sNp) wordt ontwikkeld met behulp van een microfluïdisch mengapparaat om in vitro getranscribeerd boodschapper-RNA in te kapselen en af te leveren. Het beschreven mRNA/PP-sNp kon in vitro efficiënt gekweekte cellen transfecteren.

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Cite this Article

Xiao, Q., Liu, Y., Zhang, D., Li, C., Yang, Q., Lu, D., Zhang, W., Rosenecker, J., Zou, Q., Li, Y., Guan, S. Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles. J. Vis. Exp. (186), e64288, doi:10.3791/64288 (2022).

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