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Polysome Purification from Soybean Symbiotic Nodules
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July 01, 2022
DOI:
1Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía,Universidad de la República, 2Departamento de Genómica,Instituto de Investigaciones Biológicas Clemente Estable, 3Department of Biology,University of Virginia, 4Departamento de Biología Celular y Molecular, Facultad de Ciencias,Universidad de la República
Summary
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This protocol describes a method for eukaryotic polysome purification from intact soybean nodules. After sequencing, standard pipelines for gene expression analysis can be used to identify differentially expressed genes at the transcriptome and translatome levels.
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Sainz, M. M., Filippi, C. V., Eastman, G., Sotelo-Silveira, M., Martinez, C. M., Borsani, O., Sotelo-Silveira, J. Polysome Purification from Soybean Symbiotic Nodules. J. Vis. Exp. (185), e64269, doi:10.3791/64269 (2022).
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