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Quantification of Immunostained Caspase-9 in Retinal Tissue
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05:18 min
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July 25, 2022
DOI:
1Department of Pathology & Cell Biology, Vagelos College of Physicians and Surgeons,Columbia University, 2Department of Molecular Pharmacology and Therapeutics, Vagelos College of Physicians and Surgeons,Columbia University, 3NCI-designated Cancer Center,Sanford Burnham Prebys Medical Discovery Institute, 4Department of Neurology, Vagelos College of Physicians and Surgeons,Columbia University, 5The Taub Institute for Research on Alzheimer’s Disease and the Aging Brain, Vagelos College of Physicians and Surgeons,Columbia University
Summary
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Presented here is a detailed immunohistochemistry protocol to identify, validate, and target functionally relevant caspases in complex tissues.
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Colón Ortiz, C. K., Potenski, A. M., Johnson, K. V., Chen, C. W., Snipas, S. J., Jean, Y. Y., Avrutsky, M. I., Troy, C. M. Quantification of Immunostained Caspase-9 in Retinal Tissue. J. Vis. Exp. (185), e64237, doi:10.3791/64237 (2022).
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