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小鼠星形胶质细胞的分离和直接神经元重编程

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

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小鼠星形胶质细胞的分离和直接神经元重编程

DOI:

10.3791/64175-v

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07:25 min

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July 07, 2022

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Bob A. Hersbach^2,3, Tatiana Simon, Giacomo Masserdotti²

¹Institute of Stem Cell Research, Helmholtz Zentrum München,German Research Center for Environmental Health, ²Department of Physiological Genomics, Biomedical Center Munich,Ludwig-Maximilians University, ³Graduate School of Systemic Neurosciences, BioCenter,Ludwig-Maximilians University

Capitoli

00:04Introduction
00:55Spinal Cord Tissue Dissection
01:34Magnetic Activated Cell Sorting (MACS)
02:39Seeding of Astrocytes for Reprogramming
04:25Reprogramming of Astrocytes (7‐Day Analysis)
05:29Results: Murine Astrocyte Cultures to Study Direct Neuronal Reprogramming
06:37Conclusion

Summary

Traduzione automatica

在这里，我们描述了一个详细的方案，以产生来自产后小鼠中枢神经系统不同区域的星形胶质细胞的高度富集培养物，并通过强迫转录因子将其转化为功能性神经元。

Tags

Astrocytes Neuronal Reprogramming Cell Isolation Spinal Cord Cell Culture Astrocyte Culture Poly-D-Lysine EGF BFGF

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