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Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research
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Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research
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09:28 min
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August 09, 2022
DOI:
1Leonard Davis School of Gerontology,University of Southern California, 2Molecular and Computational Biology Department,USC Dornsife College of Letters, Arts and Sciences, 3Biochemistry and Molecular Medicine Department,USC Keck School of Medicine, 4Epigenetics and Gene Regulation,USC Norris Comprehensive Cancer Center, 5USC Stem Cell Initiative
Summary
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Here we present a protocol to isolate nuclei from the brains of the short-lived vertebrate model Nothobranchius furzeri for downstream applications such as single-nucleus RNA sequencing or single-nucleus assay for transposase-accessible chromatin with sequencing (ATAC-seq).
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Teefy, B. B., Adler, A., Bhala, R., Benayoun, B. A. Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research. J. Vis. Exp. (186), e64165, doi:10.3791/64165 (2022).
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