Construction of a Human Aorta Smooth Muscle Cell Organ-On-A-Chip Model for Recapitulating Biomechanical Strain in the Aortic Wall

JoVE Journal
Bioingegneria

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JoVE Journal Bioingegneria

DOI:

10.3791/64122-v

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11:47 min

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July 06, 2022

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Mieradilijiang Abudupataer*¹, Xiujie Yin*¹, Bitao Xiang*¹, Nan Chen, Shiqiang Yan, Shichao Zhu, Yang Ming, Gang Liu, Xiaonan Zhou, Hao Lai, Chunsheng Wang, Kai Zhu, Jun Li

¹Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital,Fudan University, ²Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College,Fudan University

Capitoli

00:04Introduction
01:03Primary Human Aortic Smooth Muscle Cell Isolation
02:53Preparation of PDMS Chip
05:29PDMS Chip Surface Treatment and Sterilization
06:12Cell Seeding on the PDMS Chip and Cell Stretching Process
08:23Preparation of the Mechanical Control System
09:30Results: An HASMC‐Organ‐On‐A‐Chip Model to Precisely Control the Parameters of the Biomechanical Strain on the Aortic Wall
11:03Conclusion

Summary

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Here, we developed a human aorta smooth muscle cell organ-on-a-chip model to replicate the in vivo biomechanical strain of smooth muscle cells in the human aortic wall.

Construction of a Human Aorta Smooth Muscle Cell Organ-On-A-Chip Model for Recapitulating Biomechanical Strain in the Aortic Wall

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