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相干反斯托克斯拉曼光谱（CARS）在脑切片中成像髓鞘形成的应用

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices

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相干反斯托克斯拉曼光谱（CARS）在脑切片中成像髓鞘形成的应用

DOI:

10.3791/64013-v

•

04:08 min

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July 22, 2022

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Elizabeth A. McCullagh, Shani Poleg, Dominik Stich, Radu Moldovan*³, Achim Klug*²

¹Department of Integrative Biology,Oklahoma State University, ²Department of Physiology and Biophysics,University of Colorado Anschutz, ³Advanced Light Microscopy Core,University of Colorado Anschutz

Capitoli

00:04Introduction
01:19Staining and Imaging of the Sections
02:55Results: Analyzing Brain Tissues Using CARS Imaging
03:34Conclusion

Summary

Traduzione automatica

可视化髓鞘形成是许多研究神经系统的研究人员的重要目标。CARS是一种与免疫荧光兼容的技术，可以对组织内的脂质进行天然成像，例如大脑，照亮髓磷脂等特殊结构。

Tags

Keywords: CARS Coherent Anti-Stokes Raman Spectroscopy Myelination Myelin Lipids CH2 Bonds Microscopy Fluorescence Imaging Immunohistochemistry Multiple Sclerosis Aging Autism Laser Alignment Forward CARS Epi CARS Confocal Imaging

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