Rivista
/
Biochimica
/
Sf9纯化超加工驱动蛋白-3系列电机的单分子分析
JoVE Journal
Biochimica
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biochimica
Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors
Please note that all translations are automatically generated. Click here for the English version.

Sf9纯化超加工驱动蛋白-3系列电机的单分子分析

1,714 Views

•

08:16 min

•

July 27, 2022

DOI:

10.3791/63837-v

DOI:
10.3791/63837-v

•

08:16 min
July 27, 2022

•

2 Views
, , ,
1Discipline of Biological Engineering,Indian Institute of Technology Gandhinagar, 2Department of Biotechnology and Bioinformatics,Sambalpur University

Summary

Automatically generated

本研究详细介绍了使用Sf9杆状病毒表达系统纯化驱动蛋白-3家族成员KIF1A（1-393LZ）。这些纯化的马达的 体外 单分子和多马达滑动分析表现出与哺乳动物细胞裂解物的马达相当的稳健运动特性。因此，Sf9杆状病毒系统适合表达和纯化感兴趣的运动蛋白。

Explore More Videos

Sf9 CellsBaculovirus ExpressionSingle-molecule AnalysisKinesin-3 MotorsSuperprocessive MotorsSingle-molecule MotilityMulti-motor Gliding AssaysCytoskeletal ProteinsDionineMyosinProtein PurificationAffinity Purification

Read Article

Cite this Article

Soppina, P., Shewale, D. J., Naik, P. K., Soppina, V. Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors. J. Vis. Exp. (185), e63837, doi:10.3791/63837 (2022).

Download .ris file

Copy

Share Video

.

Copy